Study of the analytical methodologies applied to biochemistry, Quantitative biochemical measurements, Safety in the laboratory, Centrifugation (Basic principles of sedimentation, preparative and analytical centrifugation, types, care and safety aspects of centrifuges).
Molecular biology and basic techniques (Structure of nucleic acids, Genes and genome complexity, Functions of nucleic acids, The manipulation of nucleic acids – basic tools and techniques, Isolation and separation of nucleic acids, nucleic acid sequences, The polymerase chain reaction (PCR), Nucleotide sequencing of DNA, Analysing genes and gene expression, Analysing whole genomes, Pharmacogenomics.
Immunochemical techniques: Immunoassay formats, Immunoblotting, Therapeutic antibodies.
Protein structure, purification, characterisation and function analysis, ionic properties of amino acids and proteins.
Spectroscopic techniques: (Ultraviolet and visible light spectroscopy, Fluorescence spectroscopy, Luminometry, Light scattering , Atomic spectroscopy, Infrared and Raman spectroscopy, Electron paramagnetic resonance, Nuclear magnetic resonance, X-ray diffraction.
Chromatographic techniques : (Principles of chromatography, Chromatographic performance parameters,
High-performance liquid chromatography, Adsorption chromatography, Partition chromatography, Ion-exchange chromatography, Molecular (size) exclusion chromatography, Affinity chromatography, Gas chromatography.
Mass spectrometric techniques (Introduction, Ionisation, Mass analysers, Detectors, Structural information by tandem mass spectrometry, Analysing protein complexes, Computing and database analysis
Electrophoretic techniques : (General principles, Electrophoresis of proteins, Electrophoresis of nucleic acids, Capillary electrophoresis,
Omic sciences: genomics, transcriptomics, proteomics, glycomics, metabolomics, lipidomics. Definition and analysis of the Proteome, extraction of the protein from the sample, - separation by one-dimensional and or by two-dimensional electrophoresis, isoelectrofocusing, image analysis of the patterns (peptide mass fingerprint), identification of proteins in mass spectrometry.
Definition and analysis of the metabolome, metabolites (sugars, nucleotides, amino acids, lipids), approaches used in metabolomics (target analysis and non-target analysis)
Metabolomics applications in the biomedical field of: toxicology, oncology, vascular disorders.