BIOCHEMICAL METHODOLOGIES

Single discipline educational activity

Course Sheet

Academic Year of enrolment:

2016/2017

Type of Course:

Characterising

Optional/Mandatory:

Compulsory

Degree:

Master's Degree Programme in EXPERIMENTAL AND APPLIED BIOLOGY

Disciplinary Sector:

Biochemistry

Credits:

Programme Year:

Professor and Collaborators:

FORMATO Marilena

Hours of classroom activity:

Prerequisites:

Knowing the properties of biological macromolecules and their interactions.

AF code:

40000992

Assessment Methods

Verification of learning:

Written and oral, different dates

Teaching period

Second semester

Objectives

This course provides a survey of principles and purposes of a variety of biochemical methodologies devoted to the structural and functional study of biological macromolecules. The student will be able to use the logical procedures and experimental methodology that are characteristic of scientific research, illustrate the principles and purposes of some relevant molecular technologies, describe and indicate their main applications. At conclusion of the laboratory activities the student will be able to design experiments, to identify appropriate techniques for their execution, indicate possible control experiments, expose issues with scientific rigor and clarity.

Contents

Experimental method. Experimental models in biochemical research. Analytical and preparative separation techniques. Principles and applications of spectrofotometry, spectrofluorometry, ultracentrifugation, chromatography, electrophoresis, isoelectricfocusing, and capillary electrophoresis. Specific topics include two-dimensional techniques for the protein studies, differential expression proteomics and functional proteomics, image analysis, identification of protein by mass spectrometry.

Recommended Bibliography

Wilson K., Walker J. “Metodologia Biochimica” Raffaello Cortina Ed.
Alternatively
Ninfa A.J., Ballou D.P. “Metodologia di base per la biochimica e la biotecnologia” Zanichelli Ed
Reed R, Holmes D., Weyers J., Jones A. “Metodologie di base per le Scienze Biomolecolari” Zanichelli Ed.
De Marco C., Cini C. “Principi di Metodologia Biochimica” Piccin Ed
Stoppini, Bellotti "Biochimica Applicata", Edises Ed
Supplementary material (Proteomics):
Powerpoint (provided by the teacher)
Cap 12-La proteomica in biologia molecolare- in “Argomenti di Biologia Molecolare” Melino G, Ciliberto G.,Società Editrice Universo
Reccommended reading: Gorg A, Weiss W, Dunn MJ. “Current two-dimensional electrophoresis technology for proteomics”. Proteomics 2004.

Teaching Methods

Lectures and Labs (24 hours)

Contacts/More Information

Readiness to offer individual assistance in English language to incoming students; availability of supporting material (slides and selected reviews) in English language.

BIOCHEMICAL METHODOLOGIES

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BIOCHEMICAL METHODOLOGIES