The course aims to integrate the knowledge on the metabolism previously learned in the course of Biochemistry; provide the basics concerning the main preparative and analytical methods that characterize the biochemical investigation, applied to the study of proteins, enzymes and metabolites; introducing the student to perform scientific experiments, collect and present data in oral and / or written form.
Ability to apply knowledge
At the end of the course the student will have learned the principles underlying the main techniques used in biochemical investigations (centrifugation, spectrometry, electrophoresis, chromatography). This knowledge will allow him to:
- select the most suitable technique to be applied to achieve the experimental objectives defined in the research design phase;
- separate, isolate / purify and dose nucleic acids, proteins and small molecules of biological interest, in particular coenzymes, substrates and products of enzymatic reactions;
- determine the activity of an enzyme;
- evaluate the potential impact deriving from changing of the experimental conditions;
- perform the experiments independently.
Practical laboratories and classroom exercises aimed to designing and performing experimental protocols will include the use of bioinformatic tools and provide the student with the necessary skills to:
- quickly perform dilution and conversion calculations in the different measurement units / concentrations;
- appropriately approach the use of equipment (spectrophotometer, chromatographic and electrophoretic system);
- autonomously and consciously manage a work station and common material, in line with the main safety standards in the laboratory;
- to develop sufficient autonomy of judgment and evaluation of the experimental results obtained in the laboratory, thanks to the comparison with the work group and the acquired knowledge.