Part one: neuropharmacology
Introduction to the chemistry of the central nervous system.
Nerve Conduction and transmission.
Chemical synapses and electrical synapses.
Mechanisms of synaptic damage.
Brain energy metabolism and mitochondrial damage.
Neuronal antioxidant systems
Dopaminergic system and dopaminergic nerve pathways.
The nerve path of pleasure and reward.
Dopamine and feeding.
Dopamine and addictions.
Dopamine and movement.
Neurological disorders related to dopamine (Parkinson''s disease).
Psychiatric disorders linked to dopamine (Psychosis, addictions).
Serotoninic, noradrenergic transmission system and depression.
Glutametergic and GABAergic transmission system.
Epilepsy, anxiety, surgical anesthesia and drugs (antiepileptics, anxiolytics, general anesthetics).
The meaning and neurochemistry of sleep.
Nociceptive, mixed and neuropathic pain.
Brain opioidergic system and pain therapy.
Cholinergic system and Alzheimer''s disease.
Part two: neurosensors
Definition and principles of operation of neurosensors.
Microsensors and biosensors.
Classification of biosensors and main signal transduction mechanisms.
Biosensors with DNA, RNA, Aptamers.
First, second and third generation enzymatic biosensors.
Principles of Enzymology (Michaelis-Menten).
Analytical parameters of enzyme biosensors (VMAX, KM, LRS, LOF, LOQ, response time).
Principles of electrochemical signal transduction.
The potentiostat and the current-to-voltage converter.
Potentiometry and microPHmetry.
Fast-scan ciclic voltammetry.
Constant potential amperomentry.
Differential pulse voltammetry.
Permeselective non-conductive polymers (poly-OPD, poly-magnolol, poly-eugenol).
Conductive polymers (polypyrrole, PANI, PEDOT).
Enzyme stabilizers (PEI, GLYCEROL, PG).
Characteristics of biosensors for the study of in vivo neurochemistry.
Design and characterization of implantable microsensors and biosensors:
Microsensors and biosensors for oxygen.
Microsensors and biosensors for ascorbic acid.
Microsensors for nitric oxide.
Microsensors for dopamine.
Microsensors for hydrogen peroxide.
Micorsensors for total antioxidant capacity.
Biosensors for choline and acetylcholine.
Biosensors for glutamate.
Biosensors for glucose.
Biosensors for lactate.
Biosensors for ATP.
Biosensors for polyphenols.