APPLIED BIOCHEMISTRY

Single discipline educational activity

Course Sheet

Academic Year of enrolment:

2018/2019

Type of Course:

Characterising

Optional/Mandatory:

Compulsory

Degree:

5-year Single Cycle Master's Degree Programme in PHARMACEUTICAL CHEMISTRY AND TECHNOLOGY

Disciplinary Sector:

Biochemistry

Language:

Italian

Credits:

Programme Year:

Obligatory attendance:

Yes

Hours of classroom activity:

Prerequisites:

Knowing the properties of biological macromolecules and their interactions

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MEDICAL APPLIED BIOCHEMISTRY

PHARMACY

AF code:

40000833

Assessment Methods

Verification of learning:

Written test with multiple choice questions and oral exam in a different date

Teaching period

Second semester

Objectives

This course aims to raise knowledge of the principles and purposes of a variety of biochemical methodologies for the purification, identification, and characterization of biological macromolecules, and for their structural and functional study. The student will be able to use the logical procedures and experimental methodology that are characteristic of scientific research, illustrate the principles and purposes of some relevant biomolecular technologies, describe and indicate their main applications. At conclusion of the training activity the student should be able to design experiments, to identify appropriate techniques for their execution, to indicate possible control experiments, to expose issues with scientific rigor and clarity, to read and critically interpret scientific publications on the subject.

Contents

Experimental method. Experimental models in biochemical research. Safety in the laboratory. Analytical and preparative separation techniques. Principles and applications of spectrofotometry, spectrofluorometry, ultracentrifugation, chromatography, electrophoresis, isoelectricfocusing, and immunochemical techniques. Specific topics include two-dimensional techniques for the protein studies, differential expression proteomics and functional proteomics, image analysis, identification of protein by mass spectrometry, PMF, LC-MS/MS

APPLIED BIOCHEMISTRY

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APPLIED BIOCHEMISTRY