Experimental method. Experimental models in biochemical research. Safety in the laboratory. Analytical and preparative separation techniques. Principles and applications of spectrofotometry, spectrofluorometry, ultracentrifugation, chromatography, electrophoresis, isoelectricfocusing, and immunochemical techniques. Specific topics include two-dimensional techniques for the protein studies, differential expression proteomics and functional proteomics, image analysis, identification of protein by mass spectrometry, PMF, LC-MS/MS

More in detail:

Experimental method. Experimental models in biochemical research. Safety in the laboratory.
Analytical and preparative separation techniques. Protein purification for structural and functional characterization.

Centrifugation techniques: Basic principles of sedimentation, preparative and analytical ultracentrifugation, density gradient ultracentrifugation.

Spectroscopic techniques: Principles and applications of Ultraviolet and visible light spectroscopy, Fluorescence spectroscopy (FRAP, FRET), Luminometry, Light scattering (Flow cytometry, FACS), Enzyme assays.

Electrophoretic techniques : General principles, Gel Electrophoresis of proteins and nuclei acids, SDS-PAGE, Isoelectricfocusing. Capillary electrophoresis.
Proteomic analysis: Two-dimensional techniques for protein studies, differential expression proteomics and functional proteomics, image analysis, identification of proteins by mass spectrometry, peptide mass fingerprint.

Chromatographic techniques : Principles of chromatography, High-performance liquid chromatography, Adsorption chromatography, Partition chromatography, Ion-exchange chromatography, Size exclusion chromatography, Affinity chromatography. Thin layer chromatography.
High performance liquid chromatography.
Chromatographic performance parameters.

Immunochemical techniques: Immunoassay formats, western blot, ELISA, Immunoprecipitation.