General- overview of endocrinological And physiotlogical regulation of female and male reproductive activity with difference between domestic and wild species- Collection and in vitro manipulation of female gametes : methodologies and potential use in fertility preservation ,- Collection and in vitro manipulation of male gametes : methodologies and potential use in fertility preservation Cryopreservation of oocytes and embryos : technical procedures and potential use in fertility preservation programs- Cryopreservation of male gametes : technical procedures and potential use in fertility preservation programs-Controllo del ciclo ovarico, stimolazione ovarica e embryo transfer: metodologie e campi di applicazioneOvarian control, ovarian stimulation and embryo transfer: methodologies and field applications- Embryo manipulation and cloning as tool for the development of genetic resources program conservation in high endangered species- Innovative technologies in assisted reproduction : state of art e application perspectives