Basic principles: Structure and replication of DNA. RNA transcription and gene expression. Organization of the human genome. Nucleic acid purification methods. Nucleic acids storage and bio-banks organization. DNA analysis: quantity and quality parameters determined by electrophoresis and spectrophotometry. Automation in bio-banks. DNA polymorphisms: definition, differences and use of genetic markers. Enzymes in molecular biology: restriction enzymes and construction of restriction map. Hybridization techniques: RFLP analysis by Southern blotting and transcript analysis by Northern blot. Principles of PCR, its applications and problems. Diagnostic applications: PCR multiplex; Dot Blot and Reverse Dot Blot (allele-specific oligonucleotides; ASO); microsatellite analysis; STS (Sequence Tagged Site). Examples of applications. Real time PCR: differences and advantages compared to end-point PCR; methods: Taq Man, syber green, etc.; RT-PCR parameters; quantitative determinations. Applications: expression profiles and gene assay. Microarrays: principles and techniques (spotting and in situ technology), expression analysis and polymorphism detection; Data analysis for biallelic polymorphisms and expression profiles; applications and examples. DNA sequencing: Sanger''s method; advantages and problems in the use of automatic sequencers. Next generation sequencing (NGS): pyrosequencing and "sequential synthesis" method. Re-sequencing, RNA-sequencing, exome-sequencing and other NGS applications. Data analysis and troubleshooting. Overview of applications of next generation sequencing. Introduction to the management and analysis of next generation computer data (data format, analysis softwares, etc).