Assisted reproductive techniques and germplasm conservation in animal and Assisted reproductive techniques and germplasm conservation in animal and human

General Module

Course Sheet

Academic Year of enrolment:

2019/2020

Type of Course:

Characterising

Optional/Mandatory:

Compulsory

Degree:

Master's Degree Programme in HUMAN AND ANIMAL HEALTH BIOTECHNOLOGIES

Disciplinary Sector:

Clinical Veterinary Obstetrics and Gynaecology

Is part of:

REPRODUCTION BIOTECHNOLOGIES

Credits:

Programme Year:

Professor and Collaborators:

BOGLIOLO Luisa

Hours of classroom activity:

Prerequisites:

The student must have knowledge of anatomy, physiology of reproduction and cellular biology.
The student''s personal preparation, with respect to the requirements for the course, will always be checked at the beginning of the lessons

AF code:

A000355

Teaching period

First semester

Objectives

This course aims at providing detailed theoretical and methodological knowledge related to the application of assisted reproductive technologies (ARTs) in animal and human field. The students will acquire a detailed background on the structure and function of gametes, the biological and molecular mechanisms that regulate reproductive processes and on methodological approaches for gamete collection and evaluation, oocytes in vitro maturation and fertilization, gamete and embryo cryopreservation with particular reference to farm and companion animals. In addition, using gametes and embryos derived from animal models, techniques used in human assisted reproduction laboratories (PMA) will be learned.

Contents

- Overview on the anatomy of male and female reproductive organs.
- Reproductive endocrinology: hypothalamic-pituitary-gonad axis. Gonadotrophins, Steroid Hormones.
- Reproductive physiology: oogenesis and follicologenesis. Ovulation and formation of the corpus luteum. Spermatogenesis. . Reproductive cycle: estrus cycles and menstrual cycle.
Hormonal control of the ovarian cycle: Synchronization and superovulation techniques, artificial insemination (AI) and embryo transfer (ET).
-Overview of PMA techniques of 1st, 2nd and 3rd level.
-Laboratory of assisted reproduction techniques: equipment and materials.
-Ovum pick-up (OPU). Recovery of oocytes from ovaries . Qualitative evaluation of oocytes, establishment of in vitro maturation systems (IVM).
-Recovery, evaluation of semen. Establishing systems for in vitro fertilization (IVF, FIVET). Intracytoplasmic sperm injection (ICSI).
- Evaluation of the early stages of embryonic development and embryo quality. Establishment of In vitro Embryo Cultures (IVC).
Micromanipulation of oocytes and embryos; embryo splitting; cloning by nuclear transfer
- Conservation of gametes, embryos and ovarian tissue. Freezing and vitrification.

Recommended Bibliography

E.S.E. Hafez & B. Hafez. REPRODUCTION IN NUTRITION ANIMALS (Recommended chapters only).
-BIOLOGIA E TECNOLOGIE DELLA RIPRODUZIONE UMANA (TALEVI-GUALTIERI).

Assisted reproductive techniques and germplasm conservation in animal and Assisted reproductive techniques and germplasm conservation in animal and human

Course year: 1

Course year: 2

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Assisted reproductive techniques and germplasm conservation in animal and Assisted reproductive techniques and germplasm conservation in animal and human