Biochemistry
Chemical, physical and cellular fundamentals of biochemistry. Bioenergetics and Thermodynamics. Biomolecules, Water, Proteins, Carbohydrates, Lipids, Structure and functions. Cell Membranes, structure and functions. Cell metabolism, redox reactions and energy metabolism. Glycolysis, Krebs cycle, oxidative phosphorylation. Metabolism of carbohydrates, lipids, amino acids, calcium and phosphorus. Saliva and gingival fluid, etiological factors and biochemistry of caries. Cellular signals, receptors and second messengers. Signal transduction pathways, systems and techniques for the study of protein and probe for the study of cellular functions
Molecular Biology
- The structure and functions of the eukaryotic gene; the mechanisms for controlling gene expression
- Principles and Techniques of Molecular Biology: DNA and RNA extraction from biological sources (peripheral blood, cells, bacteria); restriction analysis of DNA fragments, enzymes used in molecular biology (restriction endonuclease, ligase, polymerase); polymerase chain reaction (PCR): steps of PCR; design of primers for PCR; DNA template for amplification by PCR; sensitivity and applications of PCR;
- Electrophoretic techniques: general principles, electrophoresis of nucleic acids