TECHNIQUES OF ANALYSIS OR MANIPULATION OF GENE EXPRESSION
Basic techniques in the study of gene function. Gene targeting 1.0: Genetic manipulation in animals. Transgenic mice. Site-specific recombination and inducible knock-out mice. Gene targeting 2.0: Genome-editing nuclease: ZFN, TALEN, CRISPR-Cas9. Application of the different techniques in the analysis or manipulation of gene expression.
RNA INTERFERENCE AND THERAPEUTIC RNA
RNA interference and non-coding RNA: miRNA, snoRNA, lncRNA and circRNA. Attamers. RNA splicing modifiers. Technology antimiR.
GENE THERAPY: PRINCIPLES AND APPLICATIONS
Analysis of the different viruses utilized for gene therapy: adenovirus. adeno-associated virus, retrovirus and lentivirus. Advantages and disadvantages of the different virus. Examples of gene therapy in the treatment of different human diseases.
PRECISION MEDICINE FOR NEW DIAGNOSTIC AND THERAPEUTIC APPROACHES.
General concepts. Examples of precision medicine in the treatment of different human diseases: Antibody therapies and T-CART therapy.
LABORATORY (1 ECTS):
molecular biology techniques