biochemistry and microbiology

Integrated educational activity

Course Sheet

Academic Year of enrolment:

2021/2022

Optional/Mandatory:

Compulsory

Degree:

Bachelor's Degree Programme in BIOMEDICAL LABORATORY TECHNIQUES

Language:

Italian

Moduls:

MOLECULAR BIOLOGY

2 credits
GENERAL MICROBIOLOGY

2 credits
TECHNIQUES FOR GENERAL MICROBIOLOGY

2 credits
BIOCHEMISTRY

2 credits
TECHNOLOGIES OF MOLECULAR BIOLOGY

1 credits

Credits:

Programme Year:

Hours of classroom activity:

Prerequisites:

General and inorganic chemistry, Organic Chemistry

Biology

AF code:

A000289

Assessment Methods

Verification of learning:

Test with multiple choice questions

Teaching period

Second semester

Objectives

The course aims to provide students with the physical, chemical and biological basis of biomolecules and the reactions/metabolic pathways in which they are involved. Highlight the relationship between structure and function of the major classes of macromolecules, and frame the metabolic regulation at molecular and cellular level. Contextualize the sequential connections between the various above-mentioned processes. The student will achieve knowledge on the role played by enzymatic catalysis in the phenomenon of life. The structure-function relationships of biological molecules and processes involved in their synthesis. The thermodynamic aspects of cellular metabolism and its regulation

Contents

Biochemistry
Chemical, physical and cellular fundamentals of biochemistry. Bioenergetics and Thermodynamics. Biomolecules, Water, Proteins, Carbohydrates, Lipids, Structure and functions. Myoglobin and Hemoglobin. Enzymes: Michaelis-Menten equation, enzymatic inhibition and stimulation mechanisms. Enzymes regulation in cellular metabolic pathways. Cell membranes, structure and functions. Cell metabolism, redox reactions and energy metabolism: ATP, NADH and NADPH roles. Carbohydrate metabolism: Glycolysis, Gluconeogenesis, Glycogenlysis and Glycogenesis. Pentose phosphate cycle. Krebs cycle. Lipid metabolism: beta-oxidation. Amino acid metabolism: urea cycle, electron transport chain and oxidative phosphorylation.

Molecular Biology
-Structure and functions of the eukaryotic gene; gene expression
- Principles and Techniques in Molecular Biology
- Recombinant proteins as therapeutic agents

Recommended Bibliography

1) David L. Nelson, Michael M. Cox "Lehninger Principles of Biochemistry"
2) Updated teacher's slides

Methods of Provision

Conventional

Teaching Methods

Lectures and seminars with the aid of digital slides and online materials

Contacts/More Information

Students can contact the instructors by email:
posadino@uniss.it
ccrosio@uniss.it

biochemistry and microbiology

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biochemistry and microbiology