CLINICAL BIOCHEMISTRY

Integrated educational activity

Course Sheet

Academic Year of enrolment:

2021/2022

Optional/Mandatory:

Compulsory

Degree:

Bachelor's Degree Programme in BIOMEDICAL LABORATORY TECHNIQUES

Language:

Italian

Moduls:

CLINICAL BIOCHEMISTRY

3 credits
CLINICAL MOLECULAR BIOLOGY 1

2 credits

Credits:

Programme Year:

Obligatory attendance:

Yes

Hours of classroom activity:

Prerequisites:

None

AF code:

40001080

Assessment Methods

Verification of learning:

Check the contents for each module of the Integrated Course in the corresponding sheets.

Teaching period

Second semester

Objectives

t the end of the course, the student will be able to: describe the diagnostic laboratory: to describe the diagnostic significance of the main laboratory investigations; to know the problems related to the preparation of the patient, the collection and storage of the samples; to describe the principles of analytical measurement in Clinical Biochemistry; to find the meaning and use of laboratory investigations in connection with diseases of the major organs and systems.Moreover the student will know the Clinical Molecular Biology basis, the nucleic acids properties and functions, in particularly the specific mutations and their consequences on protein function in human disease. Gene expression in prokaryotes and eukaryotes: pre transcriptional markers, post-transcriptional and post transduction. The student will be able to know : the DNA investigation strategies, and on the quality control system on the clinical molecular biology methodologies for the assessment of disease susceptibility

Contents

Introduction to Clinical Biochemistry, The biological samples: handling and storage, Analytical and biological variation, Quality control, Laboratory management.
Function and diagnostic approach of organs and tissues: kidney, heart, liver, pancreas.
Glucose metabolism and Lipid Metabolism
Introduction to the new technologies in the molecular laboratory medicine. From classic genomics to transcriptomics and proteomics. The gene expression: prokaryotes and eukaryotes, from traditional medicine to translational medicine. The epigentic phenomena: regulation and function of mammalian DNA methylation patterns: a genomic perspective. Methylation profiles of epigenome. Methods to analyze DNA methylation and their functional effects. The Biology of miRNAs: miRNA Analysis Tools, miRNA Target Prediction Tools. Expression Profiling. Prediction of novel miRNAs. General data analysis workflow of a miRNA next generation sequencing experiment. Laboratory Approaches to Mapping and Identifying Genes. From mapping to sequences.

Recommended Bibliography

Federici G. et al., Medicina di Laboratorio, ed. McGraw-Hill.
Antonozzi, Gulletta, Medicina di Laboratorio Logica e patologia Clinica Piccin
K. Wilson, J. Walker, BIOCHIMICA E BIOLOGIA MOLECOLARE. PRINCIPI E TECNICHE. Raffaello Cortina Editore.
J.C. Kaplan, M. Delpech, BIOLOGIA MOLECOLARE E MEDICINA. Casa Editrice Idelson-Gnocchi, Napoli.

Methods of Provision

Conventional

Teaching Methods

Beyond face to face lectures, students are divided in small groups to attend practical demonstrations on the main diagnostic methods of clinical biochemistry and Clinical Molecular Biology.

Contacts/More Information

Availability of the lectures slides

CLINICAL BIOCHEMISTRY

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CLINICAL BIOCHEMISTRY