A basic knowledge of General Mirobiology and Virology, cell biology, biochemistry, and molecular biology is highly recommended before entering this Course.
Evaluation will be based on student's seminars and on a practical assessment.
The aims of the Course are:
PART I, Applied Microbiology
- To gain knowledge in the field of gene cloning, with a special focus on the production of recombinant proteins in different microbial systems.
- the students will also acquire basic knowledge for a critical comprehension of genomic analysis in microbial systems.
PART II, forensic microbiology
Students will gain competences about history, current and future use of microorganisms in forensic science.
PART I: APPLIED MICROBIOLOGY
- DNA transfer in bacteria and eukaryotic cells: electroporation, transormation, transfection.
- Vectors for gene cloning and production of recombinant proteins in bacterial background. Purification of recombinant proteins.
- NGS and genome annotation
- CRISPR/CAS technology and genome editing
PART II: FORENSIC MICROBIOLOGY
- Microbiology in forensic sciences: current role and perspectives.
- Microbiology of decomposition: applications in forensics.
- Human microbiota as a fingerprint: potential use as trace evidence.
Glick, Pasternak, Patten. Molecular Biotechnology (4th ed) (ASM Press)
Carter, Tomberlin, Benbow, Metcalf. Forensic Microbiology, (Wiley)
Face-to-face and remote lectures; laboratory classes.
---- Lectures will be taught part in Italian and part in English language.