TECHNIQUES OF CELL CULTURE

General Module

Course Sheet

Academic Year of enrolment:

2013/2014

Type of Course:

Characterising

Optional/Mandatory:

Compulsory

Degree:

Bachelor's Degree Programme in BIOMEDICAL LABORATORY TECHNIQUES

Disciplinary Sector:

Laboratory Medicine Techniques

Is part of:

PHYSIOLOGY AND GENETICS

Credits:

Programme Year:

Professor and Collaborators:

DIAZ Nicia

Hours of classroom activity:

Prerequisites:

Students should already have knowledge of organic and inorganic chemistry, biochemistry and biology.

AF code:

A000283

Teaching period

Second semester

Objectives

The aim of the module is to introduce students to the practical skills base for the preparation and maintenance of cell cultures under sterile conditions.
At the end of the course the student will be able to know how to organize a laboratory cell culture, maintain a continuous cell line in culture, count and plate the known amount of cells, frozen and thawed cells, capture and present images and movies of their cells in culture.

Contents

CELL CULTURE: introduction; utility in research and diagnostics, crop contamination. Biological risk associated with cells manipulation.
STERILITY: sterile conditions for the preparation of cell cultures. Using laminar flow hoods, sterile supplies and solutions.
EQUIPMENT AND INSTRUMENTS: incubators, refrigerators / freezers +4 ° C/-20 ° C; centrifuges, phase-contrast microscope.
MEDIA AND SUPPLEMENTS: composition of the principal media, animal sera, buffers and other supplements.
PROCEDURES: Preparation of cell cultures, trypsinization and passages of cells in vitro, cell count, freezing and thawing. Cell viability: trypan blue staining and MTT assay.

Recommended Bibliography

Power Point files of the lessons
R. Ian Freshney. Culture of animal cells-A manual of basic technique. Wiley
Filippo Pasquinelli, Francesco Porta. Diagnostic and laboratory techniques. Vol 2, Masson

TECHNIQUES OF CELL CULTURE

Course year: 1

Course year: 2

Course year: 3

Search form

TECHNIQUES OF CELL CULTURE