CELL CULTURE: introduction; utility in research and diagnostics, crop contamination. Biological risk associated with cells manipulation.
STERILITY: sterile conditions for the preparation of cell cultures. Using laminar flow hoods, sterile supplies and solutions.
EQUIPMENT AND INSTRUMENTS: incubators, refrigerators / freezers +4 ° C/-20 ° C; centrifuges, phase-contrast microscope.
MEDIA AND SUPPLEMENTS: composition of the principal media, animal sera, buffers and other supplements.
PROCEDURES: Preparation of cell cultures, trypsinization and passages of cells in vitro, cell count, freezing and thawing. Cell viability: trypan blue staining and MTT assay.