Lectures (18 hours):
The in vitro culture, history (1 hour)
Organization of the laboratory, equipment and operation (3 hours)
Composition and preparation of culture substrates. Choice of the plant material (3 hours)
Sterilization of equipment. Sterilization of plant material (3 hours)
Withdrawal, multiplication subculture, rooting, adaptation to the cultivation of the soil (2 hours)
Applications (6 hours): micropropagation, callus culture, cell culture isolated; anther culture, embryo culture, culture of protoplasm; cleansing from viruses

Laboratory (38 hours):
Organization of the laboratory, equipment and its operation
Composition and preparation of substrates of culture
Choice of plant material, sterilization of plant material
Sterilization of equipment
Withdrawal, multiplication
Subculture.
rooting
Adaptation to the cultivation of the soil
Culture of callus
Culture of cells isolated
Anther culture.