The course aims to provide basic and operative knowledge in fundamental biochemical techniques such as:
- centrifugation
- radiochemical techniques
- column and thin layer chromatography
- discontinuous and gradient gel electrophoresis, isoelectrofocusing, bidimensional electrophoresis
- UV and visible spectrophotometry, particular attention on enzyme's assays and protein quantification
- Immunochemical techniques, poly- and monoclonal antibodies production, ELISA assay
- Protein purification strategies
- molecular biology; cloning, PCR, DNA sequencing, genomic DNA and cDNA libraries, recombinant proteins
