GENERAL PHYSIOLOGY
Oral examination on the program carried out in class and during exercises and laboratory hours
knowledge of the basics of general physiology is the study of fundamental properties of cells, tissues and internal environment in which one of the main organs and systems that make up the animal organism.
BODY FLUID COMPARTMENTS: osmosis; water balance; thirst center; Gibbs-Donnan equilibrium.
RENAL SYSTEM: Urine formation and concentration; renin and erythropoietin.
NERVOUS SYSTEM: excitability; physiology of neuron; action potentials; synaptic transmission; sensory receptors.
MUSCLE: molecular and mechanical aspects of muscle contraction.
BLOOD: plasma, blood cells, hemostasis and fibrinolysis.
CARDIOVASCULAR SYSTEM: function; myocardial cells; heart cycle and sounds; Frank-Starling, ECG; cardiac innervation.
RESPIRATORY SYSTEM: air environments; respiratory mechanics, lung compliance, volumes and capacities; regulation of breathing; hypoxia.
ENDOCRINOLOGY: chemical messengers; notes on chemistry, mechanism of action and function of hormons.
BERNE-LEVY: Principles of PHYSIOLOGY (Mosby)
CASELLA - TAGLIETTI: Principi di Fisiologia (La Goliardica Pavese)
SILVERTHORN: Fisiologia (Casa Ed. Ambrosiana)
GERMANN - STANFIELD: Fisiologia (EdiSES)
MARIOTTINI: Introduzione alle colture cellulari, II ed. (Tecniche nuove).
Power point of lessons face to face and esercises
Importance of in vitro cell cultures and their fields of application. Explant primary culture, strain and cell line; coltures in monolayer and in suspension; sterility; cytotoxicity and cell viability; contact inhibition; freezing techniques; cell adhesion techniques. Fixation of red cells and protein analysis by confocal microscopy, SDS-PAGE and western blotting. Determination of blood groups.
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