PART I: APPLIED MICROBIOLOGY
- DNA transfer in bacteria and eukaryotic cells: electroporation, transormation, transfection.
- Vectors for gene cloning and production of recombiannt proteins in bacterual and eukaryotic background (E.c oli, P. Pastoris, baculovirus). Purification of recombinant proteins.
- In vitro mutagenesis
- NGS and RNAseq
- Protein-protein interactions: yeast two-hybrid system.
- Recombinant antibodies: phage display.
- Genetic manipulations in plants: Agrobacterium tumefaciens.
- Biological pest control: Bacillus thuringiensis
- Applications of RNAi technology
- CRISPR/CAS technology and genome editing
PART II: APPLIED VIROLOGY
- General virology: classificaiton and structure.
- Viral genetics
- Viral zoonosis
- Pathogenesis of viral infections
- Non-conventional viruses: prion mediated diseases
- Viral diagnostics: isoaltion, cultivation and quantification of viruses. Molecular diagnosis of virus infections.
- Biotechnological applications in virology. Viral vectors for gene therapy, antiviral vaccines, viral manipulation: bioterrorism.